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Download Basic Principles in Nucleic Acid Chemistry. Volume 1 by Paul O.P. Ts'o (Eds.) PDF

By Paul O.P. Ts'o (Eds.)

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When esterase activity is assayed at different temperature, the two homozygous genotypes produce esterases which have maximum activity at opposite ends of the temperature range encountered by the species, and the heterozygous genotype produces esterase which has maximum activity at intermediate temperature. Thus, in this case, not only was the primary selective force acting on the three allozymes identified, but the physiological difference which allows selection to act was also established. The second obstacle is that we do not have sufficient knowledge of the integrated function and the total interaction of the protein in question in the living organism.

Ts'o and capacitances of about 1 fxF c m - 2 consistent with a membrane dielectric constant of 2-3. The membrane transport process in red cells of sheep, for instance, is under genetic control [124]. Research indicates that the difference in K + - N a + transport and composition (such as onabain-sensitive ATPase activity in the membrane) between two types of sheep red cells (high-potassium cell vs low-potassium cell) involves a chromosomal locus which segregates as if it were a single gene. The expression of this gene in low-potassium sheep red cells alters the kinetic properties and probably also reduces the number of K + - N a + pump sites across the membrane.

It was found that the intermediate repetitive DNA families are made up of relatively short sequences scattered widely throughout the DNA. Again, such a result of intermingling between unique sequences and repetitive sequences was also found in mouse DNA. From these observations, Britten [153] proposed the following evolutionary origin of such an arrangement: "Events of very excessive replication of short sequences occur and quite rarely the product is integrated into the genome as a cluster of tandemly organized precisely repeated sequences....

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